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KMID : 1100720120320010079
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Annals of Laboratory Medicine
2012 Volume.32 No. 1 p.79 ~ p.81
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Evaluation of a New Immunochromatographic Assay Kit for the Rapid Detection of Norovirus in Fecal Specimens
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Park Kwi-Sung
Baek Kyoung-Ah
Kim Dong-Uk
Kwon Kyung-Sook
Bing Sun-Hye
Park Joon-Soo
Nam Hae-Seon
Lee Sang-Han
Choi Young-Jin
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Abstract
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Rapid and accurate detection of norovirus is essential for the prevention and control of norovirus outbreaks. This study compared the effectiveness of a new immunochromatographic assay kit (SD BIOLINE Norovirus; Standard Diagnostics, Korea) and real-time reverse transcription-PCR (RT-PCR) for detecting norovirus in fecal specimens. Compared with real-time RT-PCR, the new assay had sensitivity, specificity, positive predictive value, and negative predictive value of 76.5% (52/68), 99.7% (342/343), 98.1% (52/53), and 95.5% (342/358), respectively. The sensitivity of the assay was 81.8% (18/22) for GII.3 and 75.7% (28/37) for GII.4. None of the 38 enteric virus-positive specimens (3 for astrovirus, 5 for enteric adenovirus, and 30 for rotavirus) tested positive in the cross-reactivity test performed by using this assay. The new immunochromatographic assay may be a useful screening tool for the rapid detection of norovirus in sporadic and outbreak cases; however, negative results may require confirmatory assays of greater sensitivity.
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KEYWORD
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Immunochromatographic assay, Norovirus, Sensitivity, Specificity
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